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PolyScience
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Journal: PLoS ONE
Article Title: Exploring Dynamics of Molybdate in Living Animal Cells by a Genetically Encoded FRET Nanosensor
doi: 10.1371/journal.pone.0058175
Figure Lengend Snippet: , Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the polyethyleneimine method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.
Article Snippet: Co-transfection of cells with pYN723 and pYN769 were performed by the polyethyleneimine transfection method using 25
Techniques: Transfection, Plasmid Preparation, Fluorescence, Expressing